解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::We have generated a monoclonal antibody (R-10G) specific to human induced pluripotent stem (hiPS)/embryonic stem (hES) cells by using hiPS cells (Tic) as an antigen, followed by differential screening of mouse hybridomas with hiPS and human embryonal carcinoma (hEC) cells. Upon western blotting with R-10G, hiPS/ES cel...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws159
更新日期:2013-03-01 00:00:00
abstract::The structure of the O-antigen polysaccharide (PS) of Escherichia coli O115 has been investigated using a combination of component analysis and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy experiments. The repeating unit of the O-antigen was elucidated using the O-deacetylated PS and has the following branc...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws161
更新日期:2013-03-01 00:00:00
abstract::Protein O-fucosyltransferase 1 (Pofut1) and protein O-fucosyltransferase 2 (Pofut2) add O-linked fucose at distinct consensus sequences in properly folded epidermal growth factor (EGF)-like repeats and thrombospondin type-1 (TSR) repeats, respectively. Glycan chain elongation past O-fucose can occur to yield a tetrasa...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws140
更新日期:2013-02-01 00:00:00
abstract::In this paper, we present two complementary strategies for enrichment of glycoproteins on living cells that combine the desirable attributes of "robust enrichment" afforded by covalent-labeling techniques and "specificity for glycoproteins" typically provided by lectin or antibody affinity reagents. Our strategy invol...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws144
更新日期:2013-02-01 00:00:00
abstract::Alg3 of Saccharomyces cerevisiae catalyzes the mannosyl transfer from Man-P-Dol to Man(5)GlcNAc(2)-PP-Dol resulting in the formation of Man(6)GlcNAc(2)-PP-Dol, which is then further processed to the final precursor oligosaccharide Glc(3)Man(9)GlcNAc(2) for N-glycosylation of proteins. Here, we identified the alg3 gene...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws135
更新日期:2013-02-01 00:00:00
abstract::Glycoprotein oligosaccharides function as tags for protein quality control in the endoplasmic reticulum (ER). Since most of proteins are glycosylated and function only after they are properly folded, glycoprotein glycan profiles in the ER might be useful to analyze various cellular status including diseases. Here, we ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws130
更新日期:2013-01-01 00:00:00
abstract::Glycosaminoglycans (GAGs) are long, anionic polysaccharides involved in many basic aspects of mammalian physiology and pathology. Here we describe a method to extract GAGs from formalin-fixed, paraffin-embedded tissues and found that they are structurally comparable with GAGs extracted from frozen tissues. We employed...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws119
更新日期:2012-12-01 00:00:00
abstract::Poly-N-acetyllactosamine extensions on N- and O-linked glycans are increasingly recognized as biologically important structural features, but access to these structures has not been widely available. Here, we report a detailed substrate specificity and catalytic efficiency of the bacterial β3-N-acetylglucosaminyltrans...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws101
更新日期:2012-11-01 00:00:00
abstract::Sialic-acid-binding immunoglobulin-like lectins (Siglecs) are a family of transmembrane receptors that are well documented to play roles in regulation of innate and adaptive immune responses. To see whether the features that define the molecular recognition of sialic acid were found in other sialic-acid-binding protei...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws073
更新日期:2012-08-01 00:00:00
abstract::Apolipoprotein C-III (apoCIII) is a small glycoprotein with a single mucin-type core-1 oligosaccharide and is analyzed by isoelectric focusing (IEF) for the diagnosis of genetic defects in O-glycan biosynthesis such as congenital disorders of glycosylation. In the present study, mass spectrometry of apoCIII, after a s...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws086
更新日期:2012-08-01 00:00:00
abstract::Bacterial protein glycosylation systems from varying species have been functionally reconstituted in Escherichia coli. Both N- and O-linked glycosylation pathways, in which the glycans are first assembled onto lipid carriers and subsequently transferred to acceptor proteins by an oligosaccharyltransferase (OTase), hav...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws059
更新日期:2012-07-01 00:00:00
abstract::Shiga toxin (Stx) 2e of Stx-producing Escherichia coli (STEC) represents the major virulence factor responsible for the pig edema disease which is characterized by hemorrhagic lesions, neurological disorders and often fatal outcomes. Stx2e-producing strains from the intestine of slaughtered pigs (n = 3), feces of pigl...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws009
更新日期:2012-06-01 00:00:00
abstract::Asparagine-linked (N-linked) protein glycosylation has been observed in all domains of life, including most recently in bacteria and is now widely considered a universal post-translational modification. However, cell-based production of homogeneous glycoproteins for laboratory and preparative purposes remains a signif...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr151
更新日期:2012-05-01 00:00:00
abstract::Glycosaminoglycan (GAG) assembly initiates through the formation of a linkage tetrasaccharide region serving as a primer for both chondroitin sulfate (CS) and heparan sulfate (HS) chain polymerization. A possible role for sulfation of the linkage structure and of the constitutive disaccharide unit of CS chains in the ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr172
更新日期:2012-04-01 00:00:00
abstract::Glycation of immunoglobulin G (IgG) can result from incubation with a reducing sugar in vitro or during circulation in vivo. Upon injection of a recombinantly produced human therapeutic IgG into humans, changes in the glycation levels could be observed as a function of circulation time. Mass changes on the individual ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr141
更新日期:2012-02-01 00:00:00
abstract::Trichomonad species are widespread unicellular flagellated parasites of vertebrates which interact with their hosts through carbohydrate-lectin interactions. In the past, some data have been accumulated regarding their lipo(phospho)glycans, a major glycoconjugate on their cell surfaces; on the other hand, other than b...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr149
更新日期:2012-02-01 00:00:00
abstract::The methylotrophic yeast, Pichia pastoris, is an important organism used for the production of therapeutic proteins. However, the presence of fungal-like glycans, such as those containing β-mannose (Man) linkages, can elicit an immune response or bind to Man receptors, thus reducing their efficacy. Recent studies have...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr108
更新日期:2011-12-01 00:00:00
abstract::Asparagine N-linked glycosylation is one of the most important forms of protein post-translational modification in eukaryotes and is one of the first metabolic pathways described at a biochemical level. Here, we report a new annotation of this pathway for the Human species, published after passing a peer-review proces...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq215
更新日期:2011-11-01 00:00:00
abstract::Different classes of glycans are implicated as mediators of apical protein sorting in the secretory pathway of epithelial cells, but recent research indicates that sorting to the apical and basolateral surfaces may occur before completion of glycan synthesis. We have previously shown that a proteoglycan (PG) core prot...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr069
更新日期:2011-11-01 00:00:00
abstract::Gangliosides (sialic acid-containing glycosphingolipids) play important roles in many physiological functions, including synaptic plasticity in the hippocampus, which is considered as a cellular mechanism of learning and memory. In the present study, three types of synaptic plasticity, long-term potentiation (LTP), lo...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr090
更新日期:2011-10-01 00:00:00
abstract::In this work, we analyzed at high resolution the sugar-binding mode of the recombinant N-terminal ricin-B domain of the hemolytic protein LSLa (LSL(150)) from the mushroom Laetiporus sulphureus and also provide functional in vitro evidence suggesting that, together with its putative receptor-binding role, this module ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr074
更新日期:2011-10-01 00:00:00
abstract::Heparan sulfate is a polysaccharide belonging to the glycaminoglycan family. It interacts with numerous proteins of the extracellular matrix, in particular cellular growth factors. The number of experimental protein-heparin sulfate complexes obtained by crystallography or nuclear magnetic resonance is limited. Alterna...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr052
更新日期:2011-09-01 00:00:00
abstract::Differences in the fertilization behavior of Xenopus borealis from X. laevis and X. tropicalis suggest differences in the glycosylation of the egg jellies. To test this assumption, O-linked glycans were chemically released from the egg jelly coat glycoproteins of X. borealis. Over 50 major neutral glycans were observe...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr003
更新日期:2011-07-01 00:00:00
abstract::The sialic acid-specific cytotoxic lectin viscumin and its recombinant equivalent rViscumin specifically bind to CD75s-gangliosides with terminal Neu5Acα6Galβ4GlcNAc sequence. We, therefore, comparatively analyzed the content of CD75s-gangliosides and closely related iso-CD75s-gangliosides (terminated by Neu5Acα3Galβ4...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq200
更新日期:2011-05-01 00:00:00
abstract::We have recently demonstrated that the mannose-binding lectins, namely banana lectin (BL) and garlic lectin (GL), interacted with the insulin receptors on M210B4 cells--an established mesenchymal cell line of murine marrow origin--and initiate mitogen-activated protein kinase kinase (MEK)-dependent extracellular signa...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq194
更新日期:2011-04-01 00:00:00
abstract::The γ-aminobutyric acid (GABA) transporters (GATs) have long been recognized for their key role in the uptake of neurotransmitters. The GAT1 belongs to the family of Na(+)- and Cl(-)-coupled transport proteins, which possess 12 putative transmembrane (TM) domains and three N-glycosylation sites on the extracellular lo...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq166
更新日期:2011-03-01 00:00:00
abstract::To date, there is no complete structural characterization of human milk glycosaminoglycans (GAGs) available nor do any data exist on their composition in bovine milk. Total GAGs were determined on extracts from human and bovine milk. Samples were subjected to digestion with specific enzymes, treated with nitrous acid,...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq164
更新日期:2011-03-01 00:00:00
abstract::Glial cell line-derived neurotrophic factor (GDNF) is a heparan sulfate (HS)-binding factor. GDNF is produced by somatic Sertoli cells, where it signals to maintain spermatogonial stem cells (SSCs) and reproduction. Here, we investigate the roles of extracellular HS 6-O-endosulfatases (Sulfs), Sulf1 and Sulf2, in the ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq133
更新日期:2011-02-01 00:00:00
abstract::In addition to sialic acid, bacteria produce several other nonulosonic acids, including legionaminic acid (Leg). This has exactly the same stereochemistry as sialic acid, with the added features of 9-deoxy and 7-amino groups. In order to explore the biological effects of replacing sialic acid residues (Neu5Ac) in glyc...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq135
更新日期:2011-01-01 00:00:00
abstract::All IgG-type antibodies are N-glycosylated in their Fc part at Asn-297. Typically, a fucose residue is attached to the first N-acetylglucosamine of these complex-type N-glycans. Antibodies lacking core fucosylation show a significantly enhanced antibody-dependent cell-mediated cytotoxicity (ADCC) and an increased effi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq109
更新日期:2010-12-01 00:00:00
abstract::In renal cell carcinoma (RCC), the presence of higher gangliosides correlates with systematic metastasis. Disialosyl globopentaosylceramide (DSGb5) was identified previously as one of the major gangliosides from RCC tissues. Siglec-7 (sialic acid-binding Ig-like lectin-7), expressed on natural killer (NK) cells as an ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq116
更新日期:2010-11-01 00:00:00
abstract::Bifidobacteria are predominant in the intestines of breast-fed infants and offer health benefits to the host. Human milk oligosaccharides (HMOs) are considered to be one of the most important growth factors for intestinal bifidobacteria. HMOs contain two major structures of core tetrasaccharide: lacto-N-tetraose (Galβ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq101
更新日期:2010-11-01 00:00:00
abstract::Although members of the serine (S)- and arginine (R)-rich splicing factor family (SR proteins) were initially purified on the basis of their splicing activity in the nucleus, there is recent documentation that they exhibit carbohydrate-binding activity at the cell surface. In contrast, galectins were isolated on the b...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwq097
更新日期:2010-10-01 00:00:00
abstract::Dendritic cell (DC) migration to secondary lymphoid organs is a critical step to properly exert its role in immunity and predominantly depends on the interaction of the chemokine receptor CCR7 with its ligands CCL21 and CCL19. Polysialic acid (PSA) has been recently reported to control CCL21-directed migration of matu...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq078
更新日期:2010-09-01 00:00:00
abstract::Stage-specific embryonic antigen-1 (SSEA-1) is a well-known carbohydrate antigenic epitope of undifferentiated cells, including neural stem cells (NSCs). However, the exact nature of the carrier proteins has not been fully characterized. Using proteomics analyses, we herein report that a lysosomal protein, LAMP-1, is ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq054
更新日期:2010-08-01 00:00:00
abstract::The endoplasmic reticulum malectin is a highly conserved protein in the animal kingdom that has no counterpart so far in lower organisms. We recently determined the structure of its conserved domain and found a highly selective binding to Glc(2)Man(9)GlcNAc(2), an intermediate of N-glycosylation. In our quest for puta...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq059
更新日期:2010-08-01 00:00:00
abstract::The evolutionarily conserved Notch signaling pathway plays broad and important roles during embryonic development and in adult tissue homeostasis. Unlike most other pathways used during animal development, Notch signaling does not rely on second messengers and intracellular signaling cascades. Instead, pathway activat...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwq053
更新日期:2010-08-01 00:00:00
abstract::Giardia lamblia, the protist that causes diarrhea, makes an Asn-linked-glycan (N-glycan) precursor that contains just two sugars (GlcNAc(2)) attached by a pyrophosphate linkage to a polyprenol lipid. Because the candidate cis-prenyltransferase of Giardia appears to be more similar to bacterial enzymes than to those of...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq036
更新日期:2010-07-01 00:00:00
abstract::Apicomplexan protozoa are a phylum of parasites that includes pathogens such as Plasmodium, the causative agent of the most severe form of malaria responsible for almost 1 million deaths per year and Toxoplasma gondii causing toxoplasmosis, a disease leading to cerebral meningitis in immunocompromised individuals or t...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwq038
更新日期:2010-07-01 00:00:00
abstract::The endoplasmic reticulum (ER) quality control system ensures that newly synthesized proteins in the early secretory pathway are in the correct conformation. Polypeptides that have failed to fold into native conformers are subsequently retrotranslocated and degraded by the cytosolic ubiquitin-proteasome system, a proc...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwq013
更新日期:2010-06-01 00:00:00